Respiration-dependent proton translocation in alkalophilic Bacillus firmus RAB and its non-alkalophilic mutant derivative.
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Purification and characterization of the cytochrome oxidase from alkalophilic Bacillus firmus RAB.
A cytochrome oxidase was purified 52-fold from membranes of alkalophilic Bacillus firmus RAB by extraction with Triton X-100, ion-exchange and hydroxyapatite chromatography, and gel filtration. On denaturing gels, the purified enzyme dissociated into two subunits of 56,000 and 40,000 Mr as well as a cytochrome c with an Mr of approximately 14,000. Heme contents calculated for an enzyme with a m...
متن کاملBioenergetic properties and viability of alkalophilic Bacillus firmus RAB as a function of pH and Na+ contents of the incubation medium.
The bioenergetic properties and viability of obligately alkalophilic Bacillus firmus RAB have been examined upon incubation in alkaline and neutral buffers in the presence or absence of added Na+. At pH 10.5, cells incubated in the absence of Na+ exhibited an immediate rise in cytoplasmic pH from less than 9.5 to 10.5, and they lost viability very rapidly. Viability experiments in the presence ...
متن کاملProton-Electrostatic Localization: Explaining the Bioenergetic Conundrum in Alkalophilic Bacteria
The decades-longstanding energetic conundrum of alkalophilic bacteria as to how they are able to synthesize ATP has now, for the first time, been clearly solved using the proton-electrostatics localization hypothesis [Bioenergetics 1:104; doi:10.4172/2167-7662.1000104]. This is a major breakthrough advance in understanding proton-coupling bioenergetics over the Nobel-prize work of Peter Mitchel...
متن کاملA sodium requirement for growth, solute transport, and pH homeostasis in Bacillus firmus RAB.
Activity of a Na+/H+ antiporter has been suggested to be critically involved in pH homeostasis in obligately alkalophilic bacteria (Krulwich, K. A., Mandel, K. G., Bornstein, R. F., and Guffanti, A. A. (1979) Biochem. Biophys. Res. Commun. 91, 58-62) and in Escherichia coli (Zilberstein, D., Padan, E., and Schuldiner, S. (1980) FEBS Lett. 116, 177-180). A concern with respect to these proposals...
متن کاملAlkalotolerant and Alkalophilic Bacteria in Seawater
Numerous alkalotolerant and alkalophilic bacteria, with concentrations ranging from 10 to 103 cells 100 ml-' water sample, were isolated from seawater in the Pacific and Indian Oceans using a buffered medium of pH 10.6. The pH effects on growth rates were used to define the occurrence of three types of alkalotolerant and alkalophilic bacterial groups: (1) bacteria exhibiting high growth rates t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)32891-6